Journal: The Japanese Dental Science Review

Article Title: Salivary SARS-CoV-2 RNA for diagnosis of COVID-19 patients: a systematic revisew and meta-analysis of diagnostic accuracy ☆

doi: 10.1016/j.jdsr.2023.06.004

Figure Lengend Snippet: Characteristics of the population on the eligible studies included.

Article Snippet: Echavarria et al. , Argentina , 174 (hospital -emergency) , 31 , , , , , , , , , , , , , , , , , , , , Symptomatic , Viral RNA detection with PCR from NPS , Quick‐RNA™ Viral Kit, Zymo Research Corp , CFX 96 Deep Well™ Real Time System (BioRad) , The E gene , Nasopharyngeal , Whole saliva.

Techniques: RNA Extraction, RNA Detection, Real-time Polymerase Chain Reaction, Purification, Diagnostic Assay, Nucleic Acid Purification, Amplification, Quantitative RT-PCR, Multiplex Assay, Reverse Transcription Polymerase Chain Reaction

Journal: Current Opinion in Environmental Science & Health

Article Title: An opinion on Wastewater-Based Epidemiological Monitoring (WBEM) with Clinical Diagnostic Test (CDT) for detecting high-prevalence areas of community COVID-19 Infections

doi: 10.1016/j.coesh.2022.100396

Figure Lengend Snippet: Salient examples of integration of wastewater monitoring with clinical testing data.

Article Snippet: Qatar , Doha 2,503,457 August 2020 , Influent WWTP Composite , PEG , RT-qPCRSARS-CoV-2(2019-nCoV)kit N1,N2 , Quick-RNA Viral Kits (Zymo Research, Irvine CA, USA Cat. No. R1041) , 7.8×10 3 -5.4× 10 5 , 500-2500 , Higher than CS , ( ) .

Techniques: Sampling, Concentration Assay, Extraction, Filtration, Centrifugation, Adsorption, Membrane, Diagnostic Assay, SYBR Green Assay, Virus, Isolation, Lysis, Variant Assay, Sequencing, RNA Extraction

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of Various Alternative Economical and High Throughput SARS-CoV-2 Testing Methods within Resource-Limited Settings

doi: 10.3390/ijms232214350

Figure Lengend Snippet: Performance of the RNA extraction methods in testing SARS-CoV-2 infection for the positive (infected) group.

Article Snippet: When comparing the cost of the Sonicator method (USD ~0.18 per sample) to the Thermo Fisher PureLink™ Kit (USD ~2.96 per sample), there was more than a 94% price reduction with Sonicator methods, making the Sonicator method the cheapest method.

Techniques: RNA Extraction, Infection

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of Various Alternative Economical and High Throughput SARS-CoV-2 Testing Methods within Resource-Limited Settings

doi: 10.3390/ijms232214350

Figure Lengend Snippet: The Ct values were compared between the internationally approved kit (Thermo Fisher PureLink™ Kit) and alternative extraction methods (Sonicator method, Bosphore EX-Tract Dry Swab RNA Solution, and Lucigen QuickExtract™ RNA Extraction Kit) for the SARS-CoV-2 target genes: N gene ( A ), S gene ( B ), Orf gene ( C ), and MS2 internal control ( D ). The results with a level of p ≤ 0.05 were considered significant. Thermo Fisher PureLink TM Kit is represented in blue, Sonicator method in pink, Bosphore EX-Tract Dry Swab RNA Solution in red, and Lucigen QuickExtract™ RNA Extraction Kit in green.

Article Snippet: When comparing the cost of the Sonicator method (USD ~0.18 per sample) to the Thermo Fisher PureLink™ Kit (USD ~2.96 per sample), there was more than a 94% price reduction with Sonicator methods, making the Sonicator method the cheapest method.

Techniques: RNA Extraction

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of Various Alternative Economical and High Throughput SARS-CoV-2 Testing Methods within Resource-Limited Settings

doi: 10.3390/ijms232214350

Figure Lengend Snippet: Comparison of the RNA extraction methods based on their simplicity cost and the observed run time for each method.

Article Snippet: When comparing the cost of the Sonicator method (USD ~0.18 per sample) to the Thermo Fisher PureLink™ Kit (USD ~2.96 per sample), there was more than a 94% price reduction with Sonicator methods, making the Sonicator method the cheapest method.

Techniques: RNA Extraction, Lysis, Binding Assay